Psmart bac vestor

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August undefined, 2024

WebCloning vector pSMART BAC v2.0 (Lucigen), no blue ones were observed, and 80% of the white colonies were proved self-ligated vectors according to the sequencing result (with … WebThe pSMART® BAC vectors incorporate transcription-free cloning to provide maximum stability. These vectors are supplied pre-cut at BamHI, EcoRI, or HindIII sites, with …

Lucigen Psmart Bac Bamhi 10 Rxns 42030-1 w/ Free Shipping

WebSteps to making a Payment and getting a Receipt. Go to eMarket. Visit PBSC eMARKET. Select the payment that applies to you. Select Checkout. Submit payment. Receipts: 2 … WebThese vectors incorporate the CloneSmart® transcription-free technology, which minimizes cloning bias and enables cloning of otherwise toxic genes, along with inducible copy number amplification (20-50X) for high DNA yields and a lacZ-sacB stuffer fragment that eliminates uncut vector from recombinants, resulting in zero background. tracking camps schwarzwald

CopyRight® v2.0 BAC Cloning Kits

WebJan 1, 2024 · To assemble the four fragments into bacterial artificial chromosome (BAC) vector, first we modified the pSMART-BAC v2.0 (Lucigen) to get ride of unwanted restriction enzyme sites and added AatII and XhoI sites to facilitate cloning by multiple rounds of fusion-PCR mediated mutagenesis. The fragments were then sequentially cloned into the … WebpSMART BAC v2.0. BAC vector with inducible copy number, suitable for cloning toxic inserts. Sequence Author: Lucigen. Open in SnapGene. Try SnapGene for Free. Download … WebCopyRight®v2.0 BAC Cloning Kit, pSMART® BAC HindIII with BAC-Optimized Replicator™v2.0 Electrocompetent Cells sufficient for 20 reactions; find Sigma-Aldrich-LUC420322 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich the rock mass

A bacterial artificial chromosome (BAC)-vectored noninfectious replicon …

Bacterial artificial chromosome - Wikipedia

WebThe pSMART® BAC vectors incorporate transcription-free cloning to provide maximum stability. These vectors are supplied pre-cut at BamHI, EcoRI, or HindIII sites, with … WebVector Database Homepage pSMART® BAC v2.0 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from … tracking candidates recruiting spreadsheetWebDec 9, 2024 · The pSMART BAC HindIII vector (7.6 kb) was fully digested and dephosphorylated by using HindIII restriction enzyme and calf intestinal alkaline phosphatase (CIAP) enzyme (NEB, UK) at 37 °C for 60 min. Afterwards, the partially digested oral metagenome was ligated into a pre-digested pSMART BAC vector by using Anza T4 … tracking canada post express

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Psmart bac vestor

Bacterial artificial chromosome - Wikipedia

http://www.biovector.net/product/138808.html WebThe new pSMART BAC vectors have a lacZ-sacB stuffer region, allowing uncut vector to be detected by blue/white screening and selected against by plating on sucrose. This feature completely eliminates uncut vector background, improving the efficiency of colony picking.

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WebApr 2, 2024 · We show that full-length cDNA clones of recent strains are most stable when a bacterial artificial chromosome is used as the vector backbone. This enabled … WebThe pSMART-BAC vector was modified as described in Materials and Methods. The full-length genomic cDNA of PEDV was amplified by RT-PCR using seven overlapping fragments designated A to G, and was assembled by using the …

WebJul 31, 2024 · The CopyRight pSMART BAC cloning vector harboring the chloramphenicol resistance gene was introduced into the AnpeNPV genome to produce the AnpeNPV bacmid that could be propagated in... WebCanvas Login. Forgot Your Password? Test Your Computer's Compatibility with Canvas.

WebApr 29, 2015 · The Lucigen pSMART BAC vector was modified to operate as a shuttle vector between E. coli and A. nidulans. The AMA1 DNA fragment, previously identified as an autonomously replicating DNA fragment from A. nidulans [ 22 ], was incorporated into pSMART BAC to create pSMART-BAC pyrGAMA1-4, or shuttle BAC vectors, and tested for … WebpSmartBAC (EU101022.1) (Search Vector Database) Backbone manufacturer Lucigen Corporation Backbone size (bp) 7648 Modifications to backbone We first restriction …

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WebShuttle BAC vector: pSMART-BAC-S pSMART BAC-S oriT rApra attP ΦC31 int tracking car for cheaper insuranceWebA similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. BACs are often used to sequence the genome of organisms in genome projects, for example the Human Genome Project. A short piece of the organism's DNA is amplified as an insert in BACs, and then sequenced. tracking candidatesWebJan 2, 2015 · The full-length RespPRRSV genome was assembled in vitro as illustrated in Fig. 1.Briefly, after gel clean-up, PCR products of both the modified pSMART BAC vector and the four viral cDNA fragments were assembled together in an isothermal GA reaction (Gibson et al., 2009).Fragments were combined with a home-made 1.3× GA master mix, … tracking cathay pacific cargoWeb8:00 AM to - 5:00 PM. View All Events. Vistamar School is a private independent high school in the South Bay region of Los Angeles. tracking carWebin BAC cloning and library construction. ˛ e pSMART® BAC or pEZ BAC vectors incorporate the CloneSmart® transcription-free technology to minimize cloning bias and enable … tracking cancer evolution through therapyWebLucigen′s CopyRight® v2.0 BAC cloning kits with pSMART® BAC or pEZ™ BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells result in unprecedented … tracking cargo vietnam airlinesWebThe CopyRight® v2.0 BAC Cloning Kits from Lucigen provide complete large-insert BAC cloning and library construction projects with unprecedented accuracy, reliability and efficiency when used with the pSMART® BAC vector and BAC-Optimized Replicator™ v2.0 Electrocompetent Cells. tracking carnival freedom cruise ship