P416tef
WebPCR products were spliced by DUG1BamHIF and DUG1HisSalIR primers and cloned at the BamHI-SalI site of p416TEF. E586A mutation was created by using the Stratagene site-directed mutagenesis kit using DUG2E586AF and DUG2E586AR primers. Mutations were confirmed by DNA sequencing. Cloning of M20A Domain of DUG2 WebWe'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2024 ...
P416tef
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WebCustom Peptide Synthesis 100% guaranteed quantity Double QC testing Fast Peptide delivery Gene Synthesis Delivery in as short as 5 days. Various vectors for selection The … Webp416TEF-Kozak-Aga2-Linker-GFP Primer Design Primers were designed for Gibson assembly of Kozak-Aga2 and GFP into the XhoI/XbaI linearized p416TEF. Just like in the …
WebMar 16, 2012 · PCR products were spliced by DUG1BamHIF and DUG1HisSalIR primers and cloned at the BamHI-SalI site of p416TEF. E586A mutation was created by using the Stratagene site-directed mutagenesis kit using DUG2E586AF and DUG2E586AR primers. Mutations were confirmed by DNA sequencing. Cloning of M20A Domain of DUG2 WebJun 3, 2011 · Plasmid p416TEF-CgCYN1 prepared from a dam −dcm− E. coli strain was digested with BclI and a HisG-Ura3-HisG cassette inserted in this site. This cassette was released from plasmid PHUKH2M (derived from pHUKH2 ( 15) and lacks the BamHI and XhoI sites), and the resulting ligation yielded p416TEF-CgCYN1M:HisG-Ura3-HisG.
WebSep 1, 2024 · The p416TEF plasmid contains a URA3 gene to allow for auxotrophic selection. URA3 encodes an orotidine 5-phosphate decarboxylase, which can also decarboxylate 5-fluorooritidine-5′-monophosphate to 5-fluorouridine-5′-monophosphate a toxic metabolite that kills cells [ 43 ]. WebYeast centromeric expression vector p416TEF Taxonomy ID: 422499 (for references in articles please use NCBI:txid422499) current name. Yeast centromeric expression vector p416TEF. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid)
WebDec 24, 2024 · Human ATP7B mRNA was cloned in p416TEF vector (as a positive control) and confirmed by sequencing. LmATP7, GFP-LmATP7, and human ATP7B constructs along with empty vector p416TEF were used to transform Wt and ccc2Δ strains. Yeast transformants were selected and maintained on SD medium without uracil (SD-Ura) at 30 …
Webp416TEF-NQYFP Plasmid #99552 Purpose Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+]. Depositor Liming Li Article Du et al Mol Cell Biol. 2010 Oct;30 (19):4644-55. Insert Swi1 NQ-rich region (AA1-536) ( SWI1 Budding Yeast) Use Tags YFP Expression Yeast Mutation Promoter TEF1 Availability Academic Institutions and Nonprofits only patch overwatch pcWebMay 1, 2024 · Glutaredoxins are small proteins of the thioredoxin superfamily that are present throughout life. Most glutaredoxins fall into two major subfamilies. Class I glutaredoxins are glutathione-dependent... pat choyWebof the constitutive TEF promoter (p416TEF and/or p413TEF vectors) were grown in selective media (SC-ura, or SC-ura-His) to exponential phase (A 600 nm 0.5–1.5). Radioactive 64Cu (specific activity of 15–30 mCi/ g of CuCl 2, Mallinckrodt Institute of Radiology at Washington University, Saint Louis) was added to 1 ml of cell culture to a final patch panel front viewWebp416TEF (Search Vector Database) Total vector size (bp) 12088 Vector type Yeast Expression Selectable markers URA3 Growth in Bacteria Bacterial Resistance (s) … tiny one wall kitchenWebtransformations. Plasmid p416TEF (CEN,AmpR URA3) was obtained from Martin Funk (19). Plasmid pHGFP-S65T was obtained from Clontech. For construction of pKNeGFP, plasmid p416TEF was mod-ified to contain the recombination domain from pOAD (13) and the eGFP coding region from pHGFP-S65T. Briefly eGFP was first am- patch panel description and functionWebThe only plasmid backbone used for expression of proteins in this part of the lab-work was p416TEF, digested with XbaI and XhoI. The plasmids consecutive promoter, TEF1, as well as terminator, CYC1, were both kept and used for the expression of our protein. The plasmid also contains the bacterial ampR gene, as well as a the yeast URA3 marker gene. tiny on tinyWebMar 1, 2024 · The p416TEF plasmid was used for plasmid construction and as the control. Δfat1 transformed with p416TEF was able to grow in the presence of α-linolenic acid, whereas the growth of Δfat1 transformed with p416TEF–CmFAX1 was significantly inhibited in a manner similar to the S. cerevisiae wild-type (Fig. 2 A, left). patchpae windows xp