Web28 nov. 2024 · One of the key enzymes involved in glycolysis is lactate dehydrogenase A (LDHA), the catalyst of the conversion of pyruvate to lactate with the oxidation of nicotinamide adenine dinucleotide dehydrogenase (NADH) to NAD + ( 11 ). Web13 jun. 2024 · Consistent with previous studies, WT Th17 cells showed increased expression of Slc2a1 (the gene encoding glucose transporter GLUT1), Ldha (lactate dehydrogenase [LDH]), and Hif1a (HIF1α) compared with naive CD4 T cells ( Fig. 3 C ). Strikingly, PKM2 deficiency did not affect the expression of those proglycolytic genes ( …
Glycolysis of Glioma Cells by Regulating the HIF ... - ResearchGate
WebLDHA and LDHB are dispensable for aerobic glycolysis in neuroblastoma cells while promoting their aggressiveness. Ždralević et al. () show that LDHA and LDHB are … Gene silencing in HUVECs was achieved by reversed transfection of siRNA directed against PFKFB3, PDHA1, LDHA, and by non-targeting siRNA (siNT) as a control (20 nM ON-TARGETplus; Dharmacon, Lafayette, CO, USA). According to the manufacturer’s protocol, siRNA was added to OPTI-MEM … Meer weergeven Primary HUVECs were isolated from umbilical cords, as described earlier69, and grown in M199 medium (Gibco, Grand Island, NY, … Meer weergeven The percentage of tip cells in cell cultures was determined as described earlier35. Briefly, HUVECs were reverse transfected with siRNA and cells were harvested by TrypLE … Meer weergeven Total ATP levels (intracellular and extracellular ATP levels) were measured using Cell titer Glo luminescent cell viability assay kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. The … Meer weergeven Total RNA was isolated using TRIzol (Invitrogen, Carlsbad, CA, USA) at 24 h after treatment, according to the manufacturer’s instructions. Briefly, total RNA was measured on a NanoDrop (ND-100; … Meer weergeven joanns gutermann thread
Regulation of glycolysis by the hypoxia‐inducible factor (HIF ...
Web1 jan. 2024 · Therefore, since LDHA is a key enzyme on the regulation of glycolysis, it plays an important role on cancer cells’ metabolism. Immunohistochemistry studies demonstrated overexpression of LDHA on cancer cells on several types of cancer, such as prostate cancer, pancreatic cancer, and breast cancer (He et al. 2015 ; Xian et al. 2015 ; … Web14 apr. 2024 · A Western blot analysis of key glycolytic pathway enzymes along with LDHA and SESN2 in HepG2 cells cultured with (+) or without (−) 4 g/L glucose for 12 h. Actin served as a loading control ... Web1 nov. 2024 · Lactate dehydrogenase (LDH) catalyses the conversion of pyruvate to lactate and NADH to NAD +; it has two isoforms, LDHA and LDHB. LDHA is a promising target … joanns ft wayne in