Chimeric repressor gene-silencing technology
WebThe Chimeric REpressor gene-Silencing Technology (CRES-T) system is a novel reverse genetic method using a chimeric transcriptional repressor fusing an EAR … WebMar 24, 2011 · Abstract: Chimeric repressor gene-silencing technology (CRES-T) is a powerful tool that has recently been developed for the functional analysis of plant transcription factors and for the genetic manipulation of plant traits. For CRES-T, a transcription factor is converted to a strong repressor by fusion with an SRDX …
Chimeric repressor gene-silencing technology
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WebChimeric REpressor gene-Silencing Technology (CRES-T) is a powerful gene-silencing tool to analyze the function of Arabidopsis transcription factors. WebOct 21, 2016 · Chimeric repressor gene silencing technology (CRES‐T) is a novel gene silencing system in which a dominant negative repressor of a TF suppresses the target genes of endogenous TF and its functionally redundant TFs (Hiratsu et al., 2003).
WebChimeric REpressor gene-Silencing Technology (CRES-T) was developed as a specific technology for gene silencing, mainly used for analyzing the function of plant … WebJun 22, 2011 · In this system, a chimeric repressor that is produced by fusion of a transcription factor to the plant-specific EAR-motif repression domain (SRDX) …
WebDec 19, 2024 · Silencing of the JA synthetic gene SlAOC results in early flower abortion, ... In addition, overexpression of AtMYB24-SRDX (a chimeric repressor of SlMYB21) in tomato delays flower opening, ... and the Beijing Natural Science Foundation project-Key Project of Science and Technology Plan of Beijing Education Commission … WebMar 10, 2014 · Arabidopsis plants transformed with a chimeric repressor for 6 transcription factors (TFs), including ADA2b, Msantd, DDF1, DREB26, AtGeBP, and ATHB23, that were converted by Chimeric REpressor gene Silencing Technology (CRES-T), show elevated salt and osmotic stress tolerance compared with wild type (WT) plants. However, the …
WebJun 2, 2016 · Chimeric REpressor gene-Silencing Technology (CRES-T) was developed as a specific technology for gene silencing, mainly used for analyzing the function of …
WebJan 30, 2024 · Introduction. Silencing gene expression, protein-coding or non-coding DNA, is a very important tool to interrogate the function of DNA. It has been reported that, with the guidance of customized single guide RNA, the catalytically inactivated dead Cas9 (dCas9) could bind to targeted promoter region to disrupt the transcriptional initiation and … ontario driver\u0027s license birthday formatWebApr 12, 2024 · Base editing, first reported in 2016, is capable of installing C•G to T•A and A•T to G•C point mutations, while largely circumventing some of the pitfalls of traditional CRISPR/Cas9 gene editing. Despite their youth, these technologies have been widely used by both academic labs and therapeutics-based companies. ontario driver\u0027s license class typesWebIn this system, a chimeric repressor that is produced by fusion of a transcription factor to the plant-specific EAR-motif repression domain (SRDX) suppresses target genes of a transcription factor dominantly over the activity of endogenous and functionally redundant transcription factors. ionabelous hotmail.comWebMay 29, 2024 · Repressor element 1 silencing transcription factor (REST) is a neuronal repressor gene that regulates neuronal stem cell differentiation [11,12]. REST also plays a multifunctional role in the regulation of non-neurogenic cells [ 13 , 14 ]. iona bercyWebJan 1, 2016 · A modified version of the EAR domain, called SRDX (LDL DLE LRL GFA), has been used to create chimeric repressor gene silencing technology (CRES-T; Mitsuda et al., 2011). SRDX has the advantage that it is a short amino acid sequence that can be fused to many different transcription factors to obtain a strong repressive form. iona basketball todayWebSep 21, 2004 · We have recently developed an effective system for gene silencing using a chimeric repressor (chimeric repressor silencing technology: the CRES-T system). In this system, a transcription factor is converted into a dominant repressor by fusion with the EAR-motif repression domain from SUPERMAN ( Hiratsu et al ., 2003 ). iona belmont gameWebMar 25, 2011 · It has previously been demonstrated that the chimeric repressor TCP3SRDX, which consists of Arabidopsis TCP3 and an ERF-associated amphiphilic … iona basketball tonight