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Bio-Rad CHEF Mapper XA System User Manual
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All-India Institute of Medical Sciences Ansari Nagar, New …
WebScreened Caps (BioRad™ 1703711) 6. Analytical balance ... 13. 20, 200 and 1000µL Micropipettes 14. BioRad™ CHEF Mapper, CHEF Mapper XA, DRII or DRIII electrophoresis system, including cooling module and pump. 3. Lysozyme (20mg/mL in TE) 4. Proteinase K (20mg/mL) 5. SeaKem® Gold (Lonza, Rockland ME)) or Agarose III WebBio-Rad CHEF Mapper XA PFGE System (Contour-clamped Homogeneous Electric Field) Theory –In an alternating electric field, larger DNA fragments take longer to re-orient and migrate more slowly Observation –Resolution of DNA fragments of up to 10 Mb WebgDNA using either the Bio-Rad CHEF Mapper XA Pulsed Field Electrophoresis or Pippin Pulse Electrophoresis Power Supply from Sage Science. Another alternative ... We show here the sizing QC of the samples on the CHEF Mapper system (Figure 3A), showing all DNA migrated predominantly above the 20 kb sizing marker. (a) 48.5 kb 22.6 kb (b) (c ... ontario black bird sk-5 knife w/ sheath